miR-34aM53
(Plasmid
#50830)
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Purposeluciferase reporter for human miR-34a
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Depositing Labs
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Depositing OrganizationArmy Medical University (Inactive)
Located in China -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50830 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 50830-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 50830-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3-Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 6084
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiR-34A promoter M53
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Alt nameMIR34A
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Alt namemiR-34A
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SpeciesH. sapiens (human)
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Insert Size (bp)1266
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Mutationp53 binding site mutated (-39)
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Entrez GeneMIR34A (a.k.a. MIRN34A, miRNA34A, mir-34, mir-34a)
- Promoter miR34a
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 50830-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 50830-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miR-34aM53 was a gift from Yun Bai & Xueqing Xu (Addgene plasmid # 50830 ; http://n2t.net/addgene:50830 ; RRID:Addgene_50830) -
For your References section:
Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells. Li J, Wang K, Chen X, Meng H, Song M, Wang Y, Xu X, Bai Y. BMC Mol Biol. 2012 Jan 31;13:4. 10.1186/1471-2199-13-4 PubMed 22292433