This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy in light of the new GDPR standards. When logging in or creating a new account, you will be asked to read and acknowledge these policy changes. Additionally, you can find our Transparency and Privacy Policy here.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #50850)


Item Catalog # Description Quantity Price (USD)
Plasmid 50850 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    residues 566–1049
  • Entrez Gene
    SOS2 (a.k.a. NS9)

Gene/Insert 2

  • Gene/Insert name
    PIF6 1-100
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
  • Tag / Fusion Protein
    • TagBFP (N terminal on insert)

Resource Information

Depositor Comments

The discrepancies between the full sequence and Addgene QC sequence do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR SFFVp BFP-PIF-SOS2cat was a gift from Wendell Lim (Addgene plasmid # 50850)
  • For your References section:

    Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Toettcher JE, Weiner OD, Lim WA. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. 10.1016/j.cell.2013.11.004 PubMed 24315106