pcDNA3.1 human cofilin S3E
Purposeexpresses psuedo-phosporylated, non-activatable cofilin in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50855||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 5912
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namecofilin 1 S3E
SpeciesH. sapiens (human)
Insert Size (bp)500
MutationS3E psuedo-phosporylated, non-activatable
Entrez GeneCFL1 (a.k.a. CFL, HEL-S-15, cofilin)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Human cofilin 1 cDNA was PCRed with a 5' primer that had an EcoRI, while the 3' primer had KpnI and EcoRV. EcoRI and KpnI were then used to ligate into pcDNA3.1- (leaving the EcoRV on the 3’ end of the insert).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 human cofilin S3E was a gift from James Bamburg (Addgene plasmid # 50855 ; http://n2t.net/addgene:50855 ; RRID:Addgene_50855)
For your References section:Cdc42 regulates cofilin during the establishment of neuronal polarity. Garvalov BK, Flynn KC, Neukirchen D, Meyn L, Teusch N, Wu X, Brakebusch C, Bamburg JR, Bradke F. J Neurosci. 2007 Nov 28;27(48):13117-29. 10.1523/JNEUROSCI.3322-07.2007 PubMed 18045906