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pET21a-Ef0113
(Plasmid #51168)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51168 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ef0113
  • Species
    Enterococcus faecalis
  • Insert Size (bp)
    624
  • Promoter T7
  • Tag / Fusion Protein
    • 6x-his (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-Ef0113 was a gift from Christopher Voigt (Addgene plasmid # 51168 ; http://n2t.net/addgene:51168 ; RRID:Addgene_51168)
  • For your References section:

    Genomic mining of prokaryotic repressors for orthogonal logic gates. Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411. 10.1038/nchembio.1411 PubMed 24316737