|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51189||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)729
- Promoter T7
/ Fusion Protein
- 6x-his (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer T7 short (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a-NonG was a gift from Christopher Voigt (Addgene plasmid # 51189 ; http://n2t.net/addgene:51189 ; RRID:Addgene_51189)
For your References section:Genomic mining of prokaryotic repressors for orthogonal logic gates. Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411. 10.1038/nchembio.1411 PubMed 24316737