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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A
(Plasmid #51248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF5-FRT-DEST
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression ; Flpin system
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gli3
  • Species
    M. musculus (mouse)
  • Mutation
    amino acids 849,865,877,907,980,1006 mutated to Ala
  • GenBank ID
    14634
  • Entrez Gene
    Gli3 (a.k.a. Bph, GLI3-190, GLI3FL, Pdn, Xt, add)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • 3xHA-TEV (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A was a gift from Rajat Rohatgi (Addgene plasmid # 51248 ; http://n2t.net/addgene:51248 ; RRID:Addgene_51248)

  • For your References section:

    Gli Protein Activity Is Controlled by Multisite Phosphorylation in Vertebrate Hedgehog Signaling. Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R. Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 10.1016/j.celrep.2013.12.003. 10.1016/j.celrep.2013.12.003 PubMed 24373970
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