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Purposecontaining 1.2 units of the HBV genome (subtype ayw)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEM-4Z
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Backbone manufacturerPromega
- Total vector size (bp) 5971
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name1.2 units of the HBV genome
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SpeciesHepatitis B virus
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GenBank IDV01460 V01460
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pQE-Fwd (5'-CCCGAAAAGTGCCACCTG-3')
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note- Plasmid 51294: HBV 1.2-mer WT replicon should be handled under BSL-2 conditions. Vaccination is highly recommended for researchers working with this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HBV 1.2-mer WT replicon was a gift from Wang-Shick Ryu (Addgene plasmid # 51294) -
For your References section:
Stimulation of hepatitis B virus genome replication by HBx is linked to both nuclear and cytoplasmic HBx expression. Cha MY, Ryu DK, Jung HS, Chang HE, Ryu WS. J Gen Virol. 2009 Apr;90(Pt 4):978-86. doi: 10.1099/vir.0.009928-0. Epub 2009 Mar 4. 10.1099/vir.0.009928-0 PubMed 19264639
Map uploaded by the depositor.
