Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pcDNA3-FLAG-Lin28A-mCCHC
(Plasmid #51372)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51372 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Lin28A
  • Alt name
    Lin28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    630
  • Mutation
    CCHC domain mutant (H147A, H169A)
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CAATGGGCGTGGATAGCG
  • 3′ sequencing primer CCAGGGTCAAGGAAGGCAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H147A, H169A

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-Lin28A-mCCHC was a gift from Narry Kim (Addgene plasmid # 51372 ; http://n2t.net/addgene:51372 ; RRID:Addgene_51372)
  • For your References section:

    Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. Heo I, Joo C, Cho J, Ha M, Han J, Kim VN. Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014. 10.1016/j.molcel.2008.09.014 PubMed 18951094