pcDNA3-V5-DGCR8
(Plasmid #51383)

• Depositing Lab: Narry Kim
• Publication: Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. Epub 2006 Sep 8.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DGCR8
• Species: H. sapiens (human)
• Insert Size (bp): 2322
• Entrez Gene: DGCR8 (a.k.a. C22orf12, DGCRK6, Gy1, pasha)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CAATGGGCGTGGATAGCG
• 3′ sequencing primer: CCAGGGTCAAGGAAGGCAC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene Quality Control sequencing has found some discrepancies in the full plasmid sequence in the V5 tag and liner region of the plasmid. The V5 tag and DGCR8 insert are not effected.