|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51483||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2400
Modifications to backboneInsertion of HygroR and peel-1
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
Growth instructionsImportant to use dual selection to limit recombination of att sites.
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See www.wormbuilder.org for updated protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ1656 was a gift from Erik Jorgensen (Addgene plasmid # 51483 ; http://n2t.net/addgene:51483 ; RRID:Addgene_51483)