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PhyB-mCherry-CAAX
(Plasmid #51567)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51567 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS305
  • Backbone size w/o insert (bp) 7236
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PhyB
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    5101
  • Mutation
    1-908aa only
  • Entrez Gene
    PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
  • Promoter ADH1pr
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • CAAX (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Apa1 (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer mCherry F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PhyB-mCherry-CAAX was a gift from Chao Tang (Addgene plasmid # 51567 ; http://n2t.net/addgene:51567 ; RRID:Addgene_51567)
  • For your References section:

    A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Yang X, Jost AP, Weiner OD, Tang C. Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub 2013 Jun 12. 10.1091/mbc.E13-03-0126 PubMed 23761071