pDEST myc MOV10 K530A
(Plasmid
#51719)
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Purposeplasmid expresses myc-tagged MOV10 K530A
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Depositing Lab
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Depositing OrganizationMax-Delbrueck-Centrum fuer Molekulare Medizin (MDC)
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51719 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 51719-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 51719-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDEST26
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5882
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMOV10 K530A
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SpeciesH. sapiens (human)
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Insert Size (bp)3012
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MutationK530A
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GenBank IDNP_001123551
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Entrez GeneMOV10 (a.k.a. fSAP113, gb110)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 51719-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 51719-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST myc MOV10 K530A was a gift from Markus Landthaler (Addgene plasmid # 51719 ; http://n2t.net/addgene:51719 ; RRID:Addgene_51719) -
For your References section:
MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs. Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa S, Dieterich C, Landthaler M. Mol Cell. 2014 May 22;54(4):573-85. doi: 10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10. 10.1016/j.molcel.2014.03.017 PubMed 24726324
Map uploaded by the depositor.
