pLpA-DN.JNK2
(Plasmid #51943)

• Purpose: Adenoviral vector for dominant negative JNK2 expression
• Depositing Lab: Jeffery Molkentin
• Publication: Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Backbone

• Vector backbone: pACCMVpLpA (pLpA)
• Backbone size w/o insert (bp): 8800
• Vector type: Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DN JNK2
• Alt name: JNK2
• Alt name: mitogen-activated protein kinase 9
• Alt name: MAPK9
• Species: M. musculus (mouse)
• Mutation: dominant negative: the dual phosphorylation motif TPY was changed to APF
• Entrez Gene: Mapk9 (a.k.a. JNK2, Prkm9, p54aSAPK)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry