|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||51948||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)rEc 27.H.Sc
LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. It has 13 "essential" genes that end in TAG recoded to TAA, and its prfA gene has been deleted. Though viable, strain growth is slow.
Genotype: ΔmutS:cat.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA, spec+
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:rEc 27.H.Sc was a gift from Farren Isaacs (Addgene plasmid # 51948)