PurposeExpresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52004||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
MutationA 2 nucleotide insertion was introduced between the FL MAVS and miniMAVS start sites
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pLXSN_5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-HA-shift-IRES-GFP(KaganE21) was a gift from Jonathan Kagan (Addgene plasmid # 52004 ; http://n2t.net/addgene:52004 ; RRID:Addgene_52004)
For your References section:A Bicistronic MAVS Transcript Highlights a Class of Truncated Variants in Antiviral Immunity. Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC. Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021. 10.1016/j.cell.2014.01.021 PubMed 24529381