|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52153||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 2280
- Total vector size (bp) 2344
Modifications to backboneReplaced the original ampicillin resistance gene Bla with the chloramphenicol resistance gene Cam from pACYC (New England Biolabs) using the Gibson Assembly method.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Copy numberHigh Copy
Gene/Insert namePumilio homology domain amino acids 133-168
Alt namePUM-HD module 4
SpeciesH. sapiens (human)
Insert Size (bp)36
Entrez GenePUM1 (a.k.a. HSPUM, PUMH, PUMH1, PUML1, SCA47)
- Cloning method Unknown
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pChlr-4NQ was a gift from Huimin Zhao (Addgene plasmid # 52153 ; http://n2t.net/addgene:52153 ; RRID:Addgene_52153)
For your References section:Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042