RCAS(A) human p110 alpha E545K
(Plasmid #52211)

• Purpose: expresses human p110 alpha with E545K mutation in avian cells
• Depositing Lab: Peter Vogt
• Publication: Kang et al Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):802-7. Epub 2005 Jan 12.

Backbone

• Vector backbone: RCAS (A)
• Backbone size w/o insert (bp): 12000
• Total vector size (bp): 15000
• Modifications to backbone: The RCAS vector is based on RCAS.Sfi, a derivative of RCAS containing SfiI(A) and SfiI(B) sites for unidirectional cloning (Aoki et al. 1998).
• Vector type: Retroviral ; avian

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p110 alpha E545K
• Alt name: E545K
• Alt name: PIK3CA E545K
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: Glutamic Acid 545 to Lysine
• Entrez Gene: PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SfiI (unknown if destroyed)
• 3′ cloning site: SfiI (unknown if destroyed)
• 5′ sequencing primer: RCAS-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry