pEL481
(Plasmid
#52351)
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PurposeGFP reporter for rack-1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepPD95.77
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Backbone manufacturerFire lab
- Backbone size w/o insert (bp) 4487
- Total vector size (bp) 8084
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert namerack-1
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SpeciesC. elegans (nematode)
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Insert Size (bp)2616
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEL481 was a gift from Erik Lundquist (Addgene plasmid # 52351 ; http://n2t.net/addgene:52351 ; RRID:Addgene_52351) -
For your References section:
RACK-1 acts with Rac GTPase signaling and UNC-115/abLIM in Caenorhabditis elegans axon pathfinding and cell migration. Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215. doi: 10.1371/journal.pgen.1001215. 10.1371/journal.pgen.1001215 PubMed 21124943
Map uploaded by the depositor.
