pME18S-13505
(Plasmid
#52384)
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PurposeAedes aegypti NPY-like receptor AAEL13505 (NPYLR1) full-length clone in mammalian expression vector
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Depositing Lab
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Depositing OrganizationRockefeller University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52384 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 52384-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 52384-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepME18S
- Backbone size w/o insert (bp) 2986
- Total vector size (bp) 4575
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNPYLR1
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Alt nameAAEL13505
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SpeciesAedes aegypti
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Insert Size (bp)1589
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GenBank IDKC439528.1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
- 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 52384-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 52384-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME18S-13505 was a gift from Leslie Vosshall (Addgene plasmid # 52384 ; http://n2t.net/addgene:52384 ; RRID:Addgene_52384) -
For your References section:
Functional and genetic characterization of neuropeptide Y-like receptors in Aedes aegypti. Liesch J, Bellani LL, Vosshall LB. PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 10.1371/journal.pntd.0002486. eCollection 2013. 10.1371/journal.pntd.0002486 PubMed 24130914
Map uploaded by the depositor.
