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pME18ST-k5310
(Plasmid #52388)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52388 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pME18S
  • Backbone size w/o insert (bp) 2986
  • Total vector size (bp) 5385
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NPYLR4
  • Alt name
    AAEL005310
  • Species
    Aedes aegypti
  • Insert Size (bp)
    2861
  • Mutation
    *
  • GenBank ID
    KC439534.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

*Note: Compared to the listed reference sequence (KC439534.1), this plasmid has an L163P amino acid residue substitution. This difference is not thought to affect plasmid function, and this plasmid accurately represents the construct as it was used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME18ST-k5310 was a gift from Leslie Vosshall (Addgene plasmid # 52388)
  • For your References section:

    Functional and genetic characterization of neuropeptide Y-like receptors in Aedes aegypti. Liesch J, Bellani LL, Vosshall LB. PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 10.1371/journal.pntd.0002486. eCollection 2013. 10.1371/journal.pntd.0002486 PubMed 24130914