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pTRE2-Hyg-omMx1
(Plasmid #52420)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52420 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 52420-D50 50 μg of DNA in Tris buffer $495
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DNA 52420-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTRE2-Hyg
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5298
  • Total vector size (bp) 7164
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)
  • Species
    Oncorhynchus mykiss
  • Insert Size (bp)
    1866
  • GenBank ID
    NM_001171901
  • Promoter TRE2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer TRE2F
  • 3′ sequencing primer TRE2R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's quality control sequencing has found two amino acid residue substitutions, G431E and M577I, compared to the depositor's full sequence. These differences are believed to be natural inter-population polymorphisms and are not thought to affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE2-Hyg-omMx1 was a gift from Bertrand Collet (Addgene plasmid # 52420 ; http://n2t.net/addgene:52420 ; RRID:Addgene_52420)

  • For your References section:

    Development of an in vitro system to measure the sensitivity to the antiviral Mx protein of fish viruses. Lester K, Hall M, Urquhart K, Gahlawat S, Collet B. J Virol Methods. 2012 Jun;182(1-2):1-8. doi: 10.1016/j.jviromet.2012.01.014. Epub 2012 Mar 1. 10.1016/j.jviromet.2012.01.014 PubMed 22405879
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