Fast-GCaMP-EF19
(Plasmid
#52644)
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Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activity
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Depositing Lab
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Depositing OrganizationPrinceton University
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 52644-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 52644-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepET28b
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Backbone manufacturerMillipore
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6722
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Modifications to backboneinserted Fast GCaMP EF19 between NotI and XbaI
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFast-GCaMP-EF19
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1353
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MutationT328D/T330D mutated from GCaMP3
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer 5'-d(TAA TAC GAC TCA CTA TAG GG)-3'
- 3′ sequencing primer 5'-d(GCT AGT TAT TGC TCA GCG G)-3
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMillipore (catalog number 69865)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 52644-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 52644-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Fast-GCaMP-EF19 was a gift from Samuel Wang (Addgene plasmid # 52644 ; http://n2t.net/addgene:52644 ; RRID:Addgene_52644) -
For your References section:
Fast GCaMPs for improved tracking of neuronal activity. Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, Hogue IB, Enquist LW, Murthy M, Wang SS. Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170. 10.1038/ncomms3170 PubMed 23863808