|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52884||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBABE hygro
- Backbone size w/o insert (bp) 5578
- Total vector size (bp) 7616
Vector typeMammalian Expression, Retroviral, Luciferase
Growth in Bacteria
Growth Strain(s)XL10 Gold
Gene/Insert namefirefly luciferase fused to IL6 3'UTR
SpeciesH. sapiens (human); firefly
Insert Size (bp)1653
Entrez GeneIL6 (a.k.a. BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE lucIL6 was a gift from Sheila Stewart (Addgene plasmid # 52884 ; http://n2t.net/addgene:52884 ; RRID:Addgene_52884)
For your References section:p38MAPK plays a crucial role in stromal mediated tumorigenesis. Alspach E, Flanagan KC, Luo X, Ruhland MK, Huang H, Pazolli E, Donlin MJ, Marsh T, Piwnica-Worms D, Monahan J, Novack DV, McAllister SS, Stewart SA. Cancer Discov. 2014 Mar 26. 10.1158/2159-8290.CD-13-0743 PubMed 24670723
Map uploaded by the depositor.