PurposeMG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + Δhfq_KanR
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||52935||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Growth Strain(s)MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + Δhfq_KanR
- Cloning method Unknown
- 5′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This strain is a precursor to HL 1188 (identical strain, except KanR cassette on chromosome allows for selection).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HL 1179 was a gift from Han Lim (Addgene plasmid # 52935)
For your References section:Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298