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pSP64-hERG1a Y652A
(Plasmid #53053)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53053 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP64
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3017
  • Total vector size (bp) 6497
  • Vector type
    Xenopus Oocyte Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Potassium voltage-gated channel subfamily H member 2
  • Alt name
    ERG1, HERG, LQT2, HERG1, Kv11.1, KCNH2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3480
  • Mutation
    changed Tyrosine 652 to Alanine
  • Entrez Gene
    KCNH2 (a.k.a. ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64-hERG1a Y652A was a gift from Michael Sanguinetti (Addgene plasmid # 53053 ; http://n2t.net/addgene:53053 ; RRID:Addgene_53053)
  • For your References section:

    A structural basis for drug-induced long QT syndrome. Mitcheson JS, Chen J, Lin M, Culberson C, Sanguinetti MC. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12329-33. 10.1073/pnas.210244497 PubMed 11005845