pSP64-hERG1a Y652A
(Plasmid #53053)

• Purpose: Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes
• Depositing Lab: Michael Sanguinetti
• Publication: Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12329-33.

Backbone

• Vector backbone: pSP64
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3017
• Total vector size (bp): 6497
• Vector type: Xenopus Oocyte Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Potassium voltage-gated channel subfamily H member 2
• Alt name: ERG1, HERG, LQT2, HERG1, Kv11.1, KCNH2
• Species: H. sapiens (human)
• Insert Size (bp): 3480
• Mutation: changed Tyrosine 652 to Alanine
• Entrez Gene: KCNH2 (a.k.a. ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1)
• Promoter: SP6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry