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pSP64-hERG1a S631A
(Plasmid #53058)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53058 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP64
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3017
  • Total vector size (bp) 6497
  • Vector type
    Xenopus Oocyte Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Potassium voltage-gated channel subfamily H member 2
  • Alt name
    ERG1, HERG, LQT2, HERG1, Kv11.1, KCNH2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3480
  • Mutation
    changed Serine 631 to Alanine
  • Entrez Gene
    KCNH2 (a.k.a. ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64-hERG1a S631A was a gift from Michael Sanguinetti (Addgene plasmid # 53058 ; http://n2t.net/addgene:53058 ; RRID:Addgene_53058)
  • For your References section:

    A mutation in the pore region of HERG K+ channels expressed in Xenopus oocytes reduces rectification by shifting the voltage dependence of inactivation. Zou A, Xu QP, Sanguinetti MC. J Physiol. 1998 May 15;509 ( Pt 1):129-37. PubMed 9547387