pSP64-mHCN2
(Plasmid #53060)

• Purpose: Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes
• Depositing Lab: Michael Sanguinetti
• Publication: Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-71.

Backbone

• Vector backbone: pSP64
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3262
• Total vector size (bp): 5854
• Vector type: Xenopus Oocyte Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
• Alt name: Hcn2; HAC1; trls; BCNG2
• Species: M. musculus (mouse)
• Insert Size (bp): 2592
• Mutation: E55G, R237H, and K283R polymorphisms compared to GenBank reference sequence
• Entrez Gene: Hcn2 (a.k.a. BCNG2, HAC1, trls)
• Promoter: SP6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry