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pSP64-mHCN2
(Plasmid #53060)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53060 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 53060-D50 50 μg of DNA in Tris buffer $495
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DNA 53060-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSP64
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3262
  • Total vector size (bp) 5854
  • Vector type
    Xenopus Oocyte Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
  • Alt name
    Hcn2; HAC1; trls; BCNG2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2592
  • Mutation
    E55G, R237H, and K283R polymorphisms compared to GenBank reference sequence
  • Entrez Gene
    Hcn2 (a.k.a. BCNG2, HAC1, trls)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64-mHCN2 was a gift from Michael Sanguinetti (Addgene plasmid # 53060 ; http://n2t.net/addgene:53060 ; RRID:Addgene_53060)

  • For your References section:

    Functional roles of charged residues in the putative voltage sensor of the HCN2 pacemaker channel. Chen J, Mitcheson JS, Lin M, Sanguinetti MC. J Biol Chem. 2000 Nov 17;275(46):36465-71. 10.1074/jbc.M007034200 PubMed 10962006

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