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Stx1a-12aa-mCer
(Plasmid #53236)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53236 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pECFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5600
  • Modifications to backbone
    This vector was created by substituting ECFP in the original vector by mCerulean. Compared to ECFP, Cerulean contains mutations: S72A, Y145A and H148D. mCerulean also contains A206K mutation to create a monomeric form of the fluorescent protein.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Syntaxin 1A
  • Alt name
    Stx1a
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    867
  • GenBank ID
    NM_053788
  • Entrez Gene
    Stx1a
  • Tags / Fusion Proteins
    • mCerulean (C terminal on backbone)
    • 12aa-linker (C terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stx1a-12aa-mCer was a gift from Robert Zucker (Addgene plasmid # 53236 ; http://n2t.net/addgene:53236 ; RRID:Addgene_53236)
  • For your References section:

    Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. Degtyar V, Hafez IM, Bray C, Zucker RS. J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013. 10.1523/JNEUROSCI.2337-12.2013 PubMed 23536066