Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #53235)


Item Catalog # Description Quantity Price (USD)
Plasmid 53235 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5333
  • Modifications to backbone
    ECFP has been substituted by citrine, which was further made monomeric by introducing the A206K mutation using the Stratagene QuikChange Site-Directed Mutagenesis Kit, and exchanged with EYFP by digestion with restriction endonucleases after ligation.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    synaptosomal-associated protein, 25kDa
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • GenBank ID
    AB003992 AB003992.1
  • Entrez Gene
    Snap25 (a.k.a. SNAP-25B, SNAP-25a)
  • Tags / Fusion Proteins
    • mCitrine (N terminal on backbone)
    • 4aa-linker (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCit-4aa-SNAP25B was a gift from Robert Zucker (Addgene plasmid # 53235 ; ; RRID:Addgene_53235)
  • For your References section:

    Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. Degtyar V, Hafez IM, Bray C, Zucker RS. J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013. 10.1523/JNEUROSCI.2337-12.2013 PubMed 23536066