Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses.
Degtyar V, Hafez IM, Bray C, Zucker RS
J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013. PubMed Article
Degtyar V, Hafez IM, Bray C, Zucker RS
J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53233 | mCer-4aa-VAMP2 | Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET. |
| 53235 | mCit-4aa-SNAP25B | Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion. |
| 53236 | Stx1a-12aa-mCer | Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers. |
| 53237 | VAMP2-12aa-mCit | Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion. |
| 53238 | mCer-Vamp2(qv/fw) | Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET. |
| 53259 | mCit-Snap25b D179K A199R | SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion. |