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mCit-Snap25b D179K A199R
(Plasmid #53259)

Full plasmid sequence is not available for this item.


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53259 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5333
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    synaptosomal-associated protein, 25kDa
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
    D179K and A199R
  • Entrez Gene
    Snap25 (a.k.a. SNAP-25B, SNAP-25a)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCitrine (N terminal on backbone)
    • 4aa-linker (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This SNAP25B double mutant is resistant to both botulinum toxin C1 (A199R) and botulinum toxin E (D179K)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCit-Snap25b D179K A199R was a gift from Robert Zucker (Addgene plasmid # 53259 ; ; RRID:Addgene_53259)
  • For your References section:

    Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. Degtyar V, Hafez IM, Bray C, Zucker RS. J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013. 10.1523/JNEUROSCI.2337-12.2013 PubMed 23536066