human CD40 promoter
(Plasmid #53433)

• Purpose: promoter activity study
• Depositing Lab: Etty Benveniste
• Publication: Nguyen et al J Biol Chem. 2002 Apr 19;277(16):13796-803. Epub 2002 Feb 5.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5597
• Total vector size (bp): 6300
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD40 promoter
• Alt name: CD40 molecule, TNF receptor superfamily member 5
• Species: H. sapiens (human)
• Insert Size (bp): 700
• GenBank ID: NM00125.4
• Entrez Gene: CD40 (a.k.a. Bp50, CDW40, TNFRSF5, p50)
• Promoter: CD40
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry