human CD40 promoter
(Plasmid
#53433)
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Purposepromoter activity study
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Depositing Lab
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Depositing OrganizationUniversity of Alabama at Birmingham Research Foundation
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 53433-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 53433-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL2 basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5597
- Total vector size (bp) 6300
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD40 promoter
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Alt nameCD40 molecule, TNF receptor superfamily member 5
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SpeciesH. sapiens (human)
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Insert Size (bp)700
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GenBank IDNM00125.4
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Entrez GeneCD40 (a.k.a. Bp50, CDW40, TNFRSF5, p50)
- Promoter CD40
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer none
- 3′ sequencing primer LucNRev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 53433-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 53433-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human CD40 promoter was a gift from Etty Benveniste (Addgene plasmid # 53433 ; http://n2t.net/addgene:53433 ; RRID:Addgene_53433) -
For your References section:
Critical role of tumor necrosis factor-alpha and NF-kappa B in interferon-gamma -induced CD40 expression in microglia/macrophages. Nguyen VT, Benveniste EN. J Biol Chem. 2002 Apr 19;277(16):13796-803. Epub 2002 Feb 5. 10.1074/jbc.M111906200 PubMed 11830590
