pMPRA1-CMV-Clover
(Plasmid
#53538)
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PurposeFluorescent reporter control for pMPRA1
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Depositing Lab
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Depositing OrganizationBroad Institute
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53538 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 53538-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 53538-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMPRA1
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Backbone manufacturerBroad Institute
- Total vector size (bp) 3973
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClover GFP
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SpeciesSynthetic; Aequorea victoria
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Insert Size (bp)717
- Promoter CMV IE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SfiI (not destroyed)
- 3′ cloning site SfiI (not destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe CMV-Clover cassette is based on Addgene Plasmid 40259 (pcDNA3-Clover) from the Michael Lin laboratory, but the physical DNA used to construct pMPRA-CMV-clover was synthesized de novo by Genscript Inc.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 53538-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 53538-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMPRA1-CMV-Clover was a gift from Tarjei Mikkelsen (Addgene plasmid # 53538 ; http://n2t.net/addgene:53538 ; RRID:Addgene_53538) -
For your References section:
Massively parallel reporter assays in cultured Mammalian cells. Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS. J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. 10.3791/51719 PubMed 25177895
Map uploaded by the depositor.
