y2
(Plasmid #53586)

• Purpose: Produces an active Arabidopsis thaliana lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E. coli.
• Depositing Lab: Francis X Cunningham Jr
• Publication: Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

Backbone

• Vector backbone: pBluescript SK-
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2958
• Total vector size (bp): 4843
• Modifications to backbone: This is an Arabidopsis thaliana cDNA library plasmid that contains an lcyE cDNA that encodes a lycopene epsilon-cyclase. The cDNA is in the reverse orientation relative to the lac promoter.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: This plasmid produces an active lycopene epsilon-monocyclase in E. coli, and can be used with pAC-LYC or pAC-LYCipi in E. coli strain TOP10 (most other E. coli strains are also suitable) to produce delta-carotene. In this case, grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness, or grow on agar plates at room temperature for 3-7 days.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lcyE
• Alt name: lycopene epsilon-cyclase
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1860
• GenBank ID: U50738.1
• Entrez Gene: LUT2 (a.k.a. AT5G57030, LUTEIN DEFICIENT 2, LYCOPENE EPSILON-CYCLASE)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry