PurposeReporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53603||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 11100
Modifications to backboneNone
Vector typeLuciferase ; reporter
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIhh promoter
SpeciesM. musculus (mouse)
Insert Size (bp)4500
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI/EcoRI (destroyed during cloning)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer Luc1
- 3′ sequencing primer NA (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIhh4.5k-Luc was a gift from Xiangli Yang (Addgene plasmid # 53603 ; http://n2t.net/addgene:53603 ; RRID:Addgene_53603)
For your References section:Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossification by activating Ihh transcription. Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, Yang X. Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub 2009 Nov 11. 10.1242/dev.043281 PubMed 19906842
Map uploaded by the depositor.