|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53620||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)Mach1 ; BL21(DE3)-R3
Copy numberHigh Copy
Insert Size (bp)439
MutationBacterial expression for structure determination; may not be full ORF, see comments
- Promoter T7
/ Fusion Protein
- His-6-TEV (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LC2/ Use BL21(DE3)-R3-pRARE2 strain for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BRPF1B-c001 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53620 ; http://n2t.net/addgene:53620 ; RRID:Addgene_53620)