|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53643||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBaculovirus expression
Growth in Bacteria
Growth Strain(s)Mach1 ; SF9
Copy numberHigh Copy
Insert Size (bp)1115
- Promoter polyhedrin
/ Fusion Protein
- His6-TEV (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. http://www.thesgc.org/structures/4CJY/ Use BL21(DE3)-R3-pRARE2 strain for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CRK7A-c021 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53643 ; http://n2t.net/addgene:53643 ; RRID:Addgene_53643)