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Addgene

pFastBac-Gm129-Flag
(Plasmid #53766)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 53766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFastbac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gm129
  • Alt name
    Ciart
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ciart (a.k.a. Chrono, Gm129)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Polyhedrin-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-Gm129-Flag was a gift from Aziz Sancar (Addgene plasmid # 53766 ; http://n2t.net/addgene:53766 ; RRID:Addgene_53766)

  • For your References section:

    Gene model 129 (Gm129) encodes a novel transcriptional repressor that modulates circadian gene expression. Annayev Y, Adar S, Chiou YY, Lieb JD, Sancar A, Ye R. J Biol Chem. 2014 Feb 21;289(8):5013-24. doi: 10.1074/jbc.M113.534651. Epub 2014 Jan 2. 10.1074/jbc.M113.534651 PubMed 24385426
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