pKSII/INSeGFP
(Plasmid #53787)

• Purpose: 1 kb zebrafish insulin promoter driving eGFP
• Depositing Lab: Larry Moss
• Publication: diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

Backbone

• Vector backbone: pBluescript II KS(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 4937
• Modifications to backbone: Zebrafish insulin promoter DNA was derived from genomic sequence, eGFP and SV40pA were inserted at engineered Nco1 site. Flanking EcoRV sites remove cassette for insertion into pTC3 expression vector (Curr Biol. 1998 Jan 15;8(2):82-8)
• Vector type: Bacterial Expression ; zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INS promoter/eGFP/pA
• Alt name: ZIP
• Species: D. rerio (zebrafish)
• Insert Size (bp): 979
• Promoter: zebrafish insulin/ZIP
• Tag / Fusion Protein:
  • eGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's quality control sequencing finds a few discrepancies with the expected sequence of this plasmid. However, the depositing laboratory believes that this plasmid accurately represents the construct as it was used in the associated publication, and it should function as described.