|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66593||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneI-Sce I element containing vector
Vector typezebrafish expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameInsulin promotor driving CFP-nitroreductase
SpeciesD. rerio (zebrafish); Aequorea victoria, E.coli
Insert Size (bp)3000
- Promoter Zebrafish Insulin promoter (approximately 1.2kb)
/ Fusion Protein
- CFP fused with N-terminus of nitroreductase (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer GCTATTCGTCCCAAAACATCTCCAC
- 3′ sequencing primer TAGCATCACAAATTTCACAAATAAAGC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ins:CFP-NTR was a gift from Didier Stainier (Addgene plasmid # 66593 ; http://n2t.net/addgene:66593 ; RRID:Addgene_66593)
For your References section:Conditional targeted cell ablation in zebrafish: a new tool for regeneration studies. Curado S, Anderson RM, Jungblut B, Mumm J, Schroeter E, Stainier DY. Dev Dyn. 2007 Apr . 236(4):1025-35. 10.1002/dvdy.21100 PubMed 17326133
Map uploaded by the depositor.