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PurposeTransgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
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Depositing Lab
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Depositing OrganizationUniversity of Vienna
Located in Austria -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 67943-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 67943-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerInvitrogen
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Vector typetransgenic reporter in the sea anemone, Nematostella vectensis
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
- Promoter Myosin Heavy Chain1 (MyHC1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer mCherry-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 67943-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 67943-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNvT-MHC::mCh was a gift from Ulrich Technau (Addgene plasmid # 67943 ; http://n2t.net/addgene:67943 ; RRID:Addgene_67943) -
For your References section:
A muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis. Renfer E, Amon-Hassenzahl A, Steinmetz PR, Technau U. Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):104-8. doi: 10.1073/pnas.0909148107. Epub 2009 Dec 14. 10.1073/pnas.0909148107 PubMed 20018670
Map uploaded by the depositor.
