mEmerald-MCM2-C-19
(Plasmid
#54163)
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PurposeLocalization: Nucleus, Excitation: 487, Emission: 509
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Depositing Lab
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Depositing OrganizationFlorida State University, Tallahassee
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54163 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 54163-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 54163-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonemEmerald-C1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCM2
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Alt nameBAA22148.1
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SpeciesM. musculus (mouse)
- Promoter CMV
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Tag
/ Fusion Protein
- mEmerald (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 487; Emission = 509. Localization data: Nucleus
DNA (Catalog # 54163-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 54163-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-MCM2-C-19 was a gift from Michael Davidson (Addgene plasmid # 54163 ; http://n2t.net/addgene:54163 ; RRID:Addgene_54163) -
For your References section:
Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload. Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally JG, Davidson MW, Gilbert DM. J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111. 10.1083/jcb.201007111 PubMed 21220507