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YFP(1-158)-beta-2 in pcDNAI/Amp
(Plasmid #54469)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54469 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNAI/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 6283
  • Modifications to backbone
    A BglII site was introduced into the polylinker of pcDNAI/Amp 3' to the BamHI site.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP(1-158)/beta-2
  • Alt name
    guanine nucleotide binding protein (G protein), beta polypeptide 2
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    1483
  • Mutation
    YFP (1-158) includes a substitution of Met for Gln-69. Gbeta-2 was amplified via PCR, which added an N-terminal linker sequence (RSIAT), a 5' BamHI site, and a 3' BglII site. Cloning into the BglII site of YFP(1-158) destroyed the BamHI site.
  • Entrez Gene
    GNB2
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP(1-158) was fused to the amino terminus of Gbeta-2. (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP(1-158)-beta-2 in pcDNAI/Amp was a gift from Catherine Berlot (Addgene plasmid # 54469 ; http://n2t.net/addgene:54469 ; RRID:Addgene_54469)
  • For your References section:

    Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot CH. J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10. 10.1074/jbc.M401432200 PubMed 15136579