|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55428||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
- Promoter CMV
/ Fusion Protein
- mCerulean3 (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
. Excitation = 433; Emission = 475
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCerulean3-Lysosomes-20 was a gift from Michael Davidson (Addgene plasmid # 55428 ; http://n2t.net/addgene:55428 ; RRID:Addgene_55428)
For your References section:An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching. Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA. PLoS One. 2011 . 6(3):e17896. 10.1371/journal.pone.0017896 PubMed 21479270