PurposeLocalization: Mannosidase/Golgi, Excitation: 462, Emission: 492
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55497||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Mutationaa 1-112 of MannII
- Promoter CMV
/ Fusion Protein
- mTFP1 (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 462; Emission = 492
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mTFP1-MannII-N-10 was a gift from Michael Davidson (Addgene plasmid # 55497 ; http://n2t.net/addgene:55497 ; RRID:Addgene_55497)
For your References section:Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE. BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. 10.1186/1741-7007-6-13 PubMed 18325109