Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #55605)


Item Catalog # Description Quantity Price (USD)
Plasmid 55605 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Dr. Bas van Steensel, Netherlands Cancer Institute
  • Backbone size w/o insert (bp) 10208
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Promoter HSP
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • DamID (DNA adenine methyltransferase identification) (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human MBD3 sequence was obtained from Thermo Scientific cDNA clone MGC:50958 IMAGE:6156267

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLgw-MBD3-V5-Dam was a gift from Paul Wade (Addgene plasmid # 55605 ; ; RRID:Addgene_55605)
  • For your References section:

    MBD3 localizes at promoters, gene bodies and enhancers of active genes. Shimbo T, Du Y, Grimm SA, Dhasarathy A, Mav D, Shah RR, Shi H, Wade PA. PLoS Genet. 2013 Dec;9(12):e1004028. doi: 10.1371/journal.pgen.1004028. Epub 2013 Dec 26. PGENETICS-D-13-01724 [pii] PubMed 24385926