Purposeexpression of mouse NRAS
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55654||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4948
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)BL21 codon plus ril de3
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneNras (a.k.a. AV095280, N-ras)
- Promoter lac
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site MfeI (not destroyed)
- 5′ sequencing primer lac promoter
- 3′ sequencing primer GST-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NRAS was a gift from Julian Downward (Addgene plasmid # 55654 ; http://n2t.net/addgene:55654 ; RRID:Addgene_55654)
For your References section:RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms. Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, Diefenbacher M, Stamp G, Downward J. Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031. 10.1016/j.cell.2013.04.031 PubMed 23706742