PurposeLocalization: Nuclear Pore, Excitation: 683, Emission: 704
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||56233||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneNUP50 (a.k.a. NPAP60, NPAP60L)
- Promoter CMV
/ Fusion Protein
- mIFP (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 683; Emission = 704
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mIFP-Nup50-N-10 was a gift from Michael Davidson (Addgene plasmid # 56233 ; http://n2t.net/addgene:56233 ; RRID:Addgene_56233)
For your References section:A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, Davidson MW, Shu X. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. 10.1038/nmeth.3447 PubMed 26098020