PurposeRiboprobe for in vitro transcription of Plexin A4
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58239||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBS KS+
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePlexin A4
SpeciesM. musculus (mouse)
Entrez GenePlxna4 (a.k.a. 9330117B14, Plxa4, mKIAA1550)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
cDNA from expressed sequence tag (EST) IMAGE clones (Invitrogen), clone ID 4316766.
For antisense RNA transcription, use SalI and T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PLEXIN A4 was a gift from Christiana Ruhrberg (Addgene plasmid # 58239 ; http://n2t.net/addgene:58239 ; RRID:Addgene_58239)
For your References section:Plexin A3 and plexin A4 convey semaphorin signals during facial nerve development. Schwarz Q, Waimey KE, Golding M, Takamatsu H, Kumanogoh A, Fujisawa H, Cheng HJ, Ruhrberg C. Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10.1016/j.ydbio.2008.08.020. Epub 2008 Sep 3. 10.1016/j.ydbio.2008.08.020 PubMed 18804103