pRSETC-RdgBbeta-splice variant 1
(Plasmid #58276)

• Purpose: bacterial expression of human RdgBbeta splice variant 1
• Depositing Lab: Shamshad Cockcroft
• Publication: Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi: 10.1042/BJ20110649.

Backbone

• Vector backbone: pRSET-C
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RdgBbeta
• Alt name: phosphatidylinositol transfer protein, cytoplasmic 1
• Species: H. sapiens (human)
• Entrez Gene: PITPNC1 (a.k.a. M-RDGB-beta, MRDGBbeta, RDGB-BETA, RDGBB, RDGBB1)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The RdgBbeta-splice variant 1 was originally obtained from Fullwood et al J Biol Chem. vol 274., p31553-31558 and matches that sequence.